Tiltle：Unravering the mechanism of biocatalysis by structural enzymology

Speaker:  Jia-Hai Zhou(Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences)

Time :  4:30 PM– 7:00 PM on July .18 2018（Monday）

Venue：E211 meeting room, Building of the College of Sciences

PROFESSIONAL EXPERIENCE

2006-Current Professor, State Key Laboratory of Bio-organic Chemistry and Natural Products

Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences,

Shanghai, China

2000-2001 Assistant Professor, State Key Laboratory of Bio-organic Chemistry and Natural Products

Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences,

Shanghai, China

1993-1995 Research Assistant, Nanjing Chemical Plant, Nanjing, Jiangsu, China

EDUCATION

1989-1993   

1995-2000  B.Sc, Analytical Chemistry, Nanjing University, China - Chemistry 

Ph.D., Bioorganic Chemistry, Shanghai Institute of Organic Chemistry, China 

2001-2006 Postdoctoral Fellow, University of Michigan, Ann Arbor, USA 

HONORS AND AWARDS

"100 Talent Program” Scholar, Chinese Academy of Sciences (2006)

Editorial Board Member, Scientific Reports (2015-Current)

RESEARCH INTEREST

The research in Zhou laboratory is mainly focused on understanding the structural basis of enzymes in biosynthetic and biocatalysis. Our approaches include a variety of biochemical and biophysical methods, particularly the high-resolution x-ray crystallography and molecular dynamics. 

SELECTED PUBLICATIONS

1. Yan Y., Liu Q., Zang X., Yuan S., Bat-Erdene1 U., Nguyen C., Gan J., Zhou J.#, Jacobsen S.E.#, Tang Y.# (2018) Resistance-Gene Directed Discovery of a Natural Product Herbicide with a New Mode of Action. Nature, doi:10.1038/s41586-018-0319-4.

2. Li Y., Zhang W., Zhang H., Tian W., Wu L., Wang S., Zheng M., Zhang J., Sun C., Deng Z., Sun Y., Qu X.#, Zhou J.# (2018) Structural Basis of a Broadly Selective Acyltransferase from the Polyketide Synthase of Splenocin. Angewandte Chemie International Edition, 57(20): 5823-5827.

3. Sun Z., Wu L., Bocola M., Chan H.C.S., Lonsdale R., Kong X.D., Yuan S.#, Zhou J.#, and Reetz M.T.# (2018) Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations. Journal of American Chemical Society 140 (1): 310–318.

4. Yuan H., Zhang J., Cai Y., Wu S., Yang K., Chan H.C.S., Huang W., Jin W.B., Li Y., Yin Y., Igarashi Y., Yuan S.#, Zhou J.# & Tang G.L.# (2017) GyrI-like proteins catalyze cyclopropanoid hydrolysis to confer cellular protection. Nature Communications 8(1):1485.

5. Li M., Zhang Z.J., Kong X.D., Yu H.L., Zhou J.# and Xu J.H.# (2017) Engineering Streptomyces coelicolor Carbonyl Reductase for Efficient Atorvastatin Precursor Synthesis. Applied and Environmental Microbiology doi:10.1128/AEM.00603-17

6. Zhang J., Liu N., Cacho R.A., Gong Z., Liu Z., Qin W., Tang C., Tang Y.#, Zhou J.# (2016) Structural basis of nonribosomal peptide macrocyclization in fungi. Nature Chemical Biology, 12:1001-1003.

7. Chen W.*, Li Y.*, Li J., Wu L., Li Y., Wang R., Deng Z.#, Zhou J.# (2016) An unusual UMP C-5 methylase in nucleoside antibiotic polyoxin biosynthesis. Protein & Cell, 7: 673-683.

8. Zhu H., Xu G., Zhang K., Kong X., Han R., Zhou J.#, Ni Y.# (2016) Crystal structure of tyrosine decarboxylase and key residues in conformational swing and substrate binding. Scientific Reports, 6:27779. 

9. Sun Z., Lonsdale R., Wu L., Li G., Li A., Wang J., Zhou J.#, Reetz M.T.# (2016) Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase.  ACS Catalysis, 6:1590-1597.

10. Sun Z., Lonsdale R., Kong X.D., Xu J.H., Zhou J.#, Reetz M.T.# (2015) Reshaping an enzyme binding pocket for enhanced and inverted stereoselectivity: use of smallest amino acid alphabets in directed evolution. Angewandte Chemie International Edition, 54: 12410-12415.

11. Kong X.D., Yuan S., Li L., Chen S., Xu J.H. #, Zhou J. # (2014) Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates. Proceedings of the National Academy of Sciences USA, 111: 15717-15722.

12. Kong, X.D., Ma Q., Zhou J.#, Zeng, B.B.#, Xu J.H.# (2014) A smart library of epoxide hydrolase variants with best hits for synthesis of (S)-β-blocker precursors. Angewandte Chemie International Edition, 53: 6641-6644. 

13. Luo, X.J., Kong X.D., Zhao J., Chen Q., Zhou J.H.#, Xu J.H.#  (2014) Switching a newly discovered lactonase into an efficient and thermostable phosphotriesterase by simple double mutations His250Ile/Ile263Trp. Biotechnology and Bioengineering, 111: 1920-1930.

14. Li J.*, Wang C.*, Zhang Z.M., Cheng Y.Q.#, Zhou J.# (2014) The structural basis of an NADP+-independent dithiol oxidase in FK228 biosynthesis. Scientific Reports, 4: 4145.

15. Zhang Z.M.*, Yang F.*, Zhang J., Tang Q., Li J., Gu J., Zhou J.#, Xu Y.Z. # (2013) Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly. Cell Reports, 5(5): 1269-1278. 

16. Xu Z., Li, S., Li J., Li Y., Feng X., Wang R., Xu H. #, Zhou J. # (2013) The structural basis of Erwinia rhapontici isomaltulose synthase. PLoS ONE, 8(9): e74788. 

17. Ma J. *, Wu L. *, Guo F., Gu J., Tang X., Jiang L., Liu J., Zhou J.#, Yu H#. (2013) Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution. Applied Microbiology and Biotechnology, 97(11): 4897-4906. 

18. Sun Y. *, Song H. *, Li J., Li Y., Jiang M., Zhou J. #, Guo Z. # (2013) Structural basis of the induced-fit mechanism of 1,4-dihydroxy-2-naphthoyl coenzyme A synthase from the crotonase fold superfamily. PLoS ONE, 8(4): e63095. 

19. Zhang Z.M. *, Zhang X. *, Zhou Z.R., Hu H.Y., Liu M., Zhou B.#, Zhou J.#. (2013) NMR Structure Note: Solution structure of the Magnaporthe oryzae avirulence protein AvrPiz-t. Journal of Biomolecular NMR, 55(2): 219-223. 

20. Sun Y., Song H., Li J., Jiang M., Li Y., Zhou J.#, Guo Z.# (2012) Active site binding and catalytic role of bicarbonate in 1,4-dihydroxy-2-naphthoyl coenzyme A synthases from vitamin K biosynthetic pathways. Biochemistry, 51(22): 4580-4589. 

21. Diao L., Dong Q., Xu Z., Yang S., Zhou J.#, and Freudl R.# (2012) Functional implementation of the posttranslational SecB-SecA protein targeting pathway in Bacillus subtilis. Applied and Environmental Microbiology, 78(3): 651-659.

22. Zhou J.*, Liu C.Y.*., Back S.H., Clark R.L., Peisach D., Xu Z. and Kaufman R.J., (2006) The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of unfolded protein response.  Proceedings of the National Academy of Sciences USA, 103(39): 14343-14348.

23. Zhou J., Xu Z. (2005) The structural view of bacterial translocation-specific chaperone SecB: implications for function, Molecular Microbiology, 58(2): 349-357.

24. Zhou J., Xu Z. (2003) Structural determinants of SecB recognition by SecA in bacterial protein translocation, Nature Structural Biology, 10(11): 942-947.

25. Wang Y.*, Zhou J.*, Wang Y., Bao J., Chen H. (2001) Properties of hybrid enzymes between Synechococcus large subunits and higher planet small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase in Escherichia coli, Archives of Biochemistry and Biophysics, 396(1): 35-42. 

* same contribution, # co-corresponding author